Engineered microdevices for single cell immunological assay

Microdevices for examining immunological responses of single cells to HIV

More than 60 million people in the world have been diagnosed with HIV infections since the virus was recognized as the causative agent of AIDS in the 1980s. Even though more than half of the infected patients have died, effective disease treatment and prevention measures have not been established. ART (antiretroviral therapy) is the only proven HIV treatment that sustains the suppression of pat...

Immunological properties of engineered nanomaterials.

Most research on the toxicology of nanomaterials has focused on the effects of nanoparticles that enter the body accidentally. There has been much less research on the toxicology of nanoparticles that are used for biomedical applications, such as drug delivery or imaging, in which the nanoparticles are deliberately placed in the body. Moreover, there are no harmonized standards for assessing th...

Disposable Microdevices for Dna Analysis and Cell Sorting

We have developed microfabricated devices to size and sort microscopic objects, based on measurement of fluorescent properties. With these devices, we have demonstrated sizing and sorting on various biological entities, ranging from E. Coli cells to single molecules of DNA. The microfabricated devices have several advantages over macroscopic systems, including size, cost and sensitivity. For ex...

Single Cell Gell Electrophoresis (Comet Assay)

AgentCell: a digital single-cell assay for bacterial chemotaxis

MOTIVATION In recent years, single-cell biology has focused on the relationship between the stochastic nature of molecular interactions and variability of cellular behavior. To describe this relationship, it is necessary to develop new computational approaches at the single-cell level. RESULTS We have developed AgentCell, a model using agent-based technology to study the relationship between ...